The heterologous expression of the indole degradation gene cluster in Escherichia coli BL21 (DE3) and in vitro enzymatic reaction demonstrated that the indole biodegradation gene cluster may contribute to selectively biosynthesizing Tyrian purple. An indole degradation gene cluster for indole metabolism was identified from this GS-2 strain.
This GS-2 strain was then identified as Providencia rettgeri based on bacterial genome sequencing analysis. In this study, we found Tyrian purple can be selectively produced by a bacterial strain GS-2 when fed with 6-bromotryptophan in the presence of tryptophan. Tyrian purple, mainly composed of 6, 6′-dibromoindigo, is a precious dye extracted from sea snails. 4Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.3CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.2Shenzhen Key Laboratory for the Intelligent Microbial Manufacturing of Medicines, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.1School of Life Sciences, Inner Mongolia University, Hohhot, China.Writer(s): Max Ten OeverLyrics powered by Li 1,2,3,4, Huaxiang Deng 2,3,4, Biming Zhong 2,3,4, Banlai Ruan 2,3,4, Xixi Zhao 2,3,4* and Xiaozhou Luo 1,2,3,4* I′ve been floating I've been laughing Want to see through the troubles tonight On the big hill With the flowers Always greener through the branches somehow Climbing higher To the top now Balance my feet on the spike Sturdy wooden soles Pointy figures In the distance Purple cloaks and yielding mace Gates are closed to keep them safe Let′s dive to the bottom of a Body of blue water see the Grainy floor is lava keeps me Nice and warm Tyrian what I'm in search for Snail who tries to hide I'll ignore Stick others to my uniform Pigments oohoohooo Please tell them all to wait (No purple around us) It needs patience to create (Or no more purple around us) Now there′s no purple around us Just empty shells (shells!) You′d never tell (tell!) There was a difference So much it spills from the roof tops Straight to the wells (wells!) I catch myself (self!) Almost drinking from them Now there's no purp Now there′s no purp Now there's no purp lee Something no one wished for Now there′s no purp Now there's no purp Now there′s no purp lee Something no one wished for I've been floating I've been laughing Want to see through the troubles tonight On the big hill With the flowers Always greener through the branches somehow
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